Site-Directed Mutagenesis by Fusion of Contiguous DNA Fragments
نویسندگان
چکیده
منابع مشابه
Site-directed mutagenesis by fusion of contiguous DNA fragments.
Efficient site-directed mutagenesis (SDM) is a powerful tool for the analysis of the function of DNA sequences. The polymerase chain reaction (PCR) (4,9) and commercially available methods for in vitro selection of mutated unmethylated DNA (Stratagene, La Jolla, CA, USA) have been used increasingly as rapid alternatives to classical methods of site-specific mutation (11). Generating mutations a...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99263bm02